In vitro ADME

Metabolic Stability and Metabolite Identification

• Liver microsomes
• Human Hepatocytes
• S9 fractions
• Plasma and whole blood
• Metabolic pathway and metabolites identification

Drug-Drug Interactions

• CYP450 inhibition:
• CYP450 induction
• CYP450 phenotyping

Distribution

• Plasma protein binding
• Blood partitioning
• Caco-2 Permeability studies
• MDR1-MDCK effective efflux

Physical Chemical Properties

• Solubility and Log D
• Chemical stability

In Vitro Toxicity

• Non-GLP Mini-Ames test
• hERG

At ChemPartner, we know that rapid turnaround of data generated from in vitro ADME studies is critical to making informed decisions on the drug-like properties of new molecular entities. We offer state-of-the-art instrumentation with supporting bioanalytical laboratories and a broad range of in vitro ADME assays.

Broad Range of In-Vitro ADME Assays

• Physico-chemical: Kinetic and thermo solubility, Log P, Log D, and pKa

• Absorption: Caco-2 and MDCK-MDR1, MDCK-BCRP and MDCK-MDR1-BCRP
  • Cell Based assays to look at either passive and active absorption, or efflux characteristics
• Metabolism: Liver microsomes, S9 fraction, or hepatocytes to determine the half-life or rate of disappearance of parent molecule over time
  • Metabolism: Liver microsomes, S9 fraction, or hepatocytes to determine the half-life or rate of disappearance of parent molecule over time

• Drug-Drug Interactions: CYP P450 Inhibition, Induction, time dependent inhibition and phenotyping assays to identify compound liabilities and identify potential for drug-drug interactions prior to development

• Distribution: Protein binding using equilibrium dialysis or ultracentrifugation to look at % bound of molecules

• In Vitro Tox: hERG and mini-Ames or regular AMES testing