In vitro ADME
Metabolic Stability and Metabolite Identification
- Liver microsomes
- Human Hepatocytes
- S9 fractions
- Plasma and whole blood
- Metabolic pathway and metabolites identification
Drug-Drug Interactions
- CYP450 inhibition:
- CYP450 induction
- CYP450 phenotyping
Distribution
- Plasma protein binding
- Blood partitioning
- Caco-2 Permeability studies
- MDR1-MDCK effective efflux
Physical Chemical Properties
- Solubility and Log D
- Chemical stability
In Vitro Toxicity
- Non-GLP Mini-Ames test
- hERG
At ChemPartner, we know that rapid turnaround of data generated from in vitro ADME studies is critical to making informed decisions on the drug-like properties of new molecular entities. We offer state-of-the-art instrumentation with supporting bioanalytical laboratories and a broad range of in vitro ADME assays.
Broad Range of In-Vitro ADME Assays
- Physico-chemical: Kinetic and thermo solubility, Log P, Log D, and pKa
- Absorption: Caco-2 and MDCK-MDR1, MDCK-BCRP and MDCK-MDR1-BCRP
- Cell Based assays to look at either passive and active absorption, or efflux characteristics
- Metabolism: Liver microsomes, S9 fraction, or hepatocytes to determine the half-life or rate of disappearance of parent molecule over time
- Metabolism: Liver microsomes, S9 fraction, or hepatocytes to determine the half-life or rate of disappearance of parent molecule over time
- Drug-Drug Interactions: CYP P450 Inhibition, Induction, time dependent inhibition and phenotyping assays to identify compound liabilities and identify potential for drug-drug interactions prior to development
- Distribution: Protein binding using equilibrium dialysis or ultracentrifugation to look at % bound of molecules
- In Vitro Tox: hERG and mini-Ames or regular AMES testing
- Service Overview
- Discovery Chemistry
- Biology & Pharmacology
- DMPK / Exploratory Toxicology
- Biologics Discovery
- Biologics CMC
- Small Molecule CMC