Epigenetics
The molecular basis of epigenetics, the study of heritable phenotype changes that do not involve alterations in the DNA sequence, mainly includes the modification of DNA and histones, which lead to the up-regulation or down-regulation of the related gene expression....
Kinases
Kinases play essential roles in cell signal transduction, thereby regulating various physiological functions of cells; therefore, kinases are an essential drug target for many diseases. ChemPartner’s in vitro biology team has established nearly 200 kinase assays by...
Other Cell Assay Examples
PROTAC – Detect Target Protein Degradation by Western Blot, ELISA, and HTRF Chemical Degrader Western Blot ELISA HTRF
Cancer Cell Line Panel Screen
800+ Cancer Cell Line Collection for Anti-proliferation Panel Screening >80% of the cell lines are in CCLE panel with genetic annotationsRepresentative of genomic diversity, tissue, and ethnic originsHigh-quality cell panel guaranteed by STR verification and...
Cell Signaling Assays
Protein Phosphorylation Western blot Cell-based ELISA AlphaScreen SureFire High content analysis NanoBRET FACS Nuclear Translocation Reporter Gene Assay p-Akt Detected by AlphaScreen SureFire AlphaScreen SureFire Technology pAKT473 in Suspension Cells by AlphaScreen...
Cell Functional Assays
Cell Proliferation/Viability CellTiter-Glo cell viability assay CyQuant assay MTT/MTS assays Direct cell counting by HCA Clonogenic assay 14-day long-term proliferation assay 3D Growth Soft agar colony formation assay Matrigel 3D assay Ultra-low attachment...
CRISPR/Cas9 Mediated Target Gene Knockout
Validation of Cas9 Expression by Western Blot Validation of Cas9 Expression by FACS Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection) CRISPR Target Gene Knockout – Phenotypic Study Knockout of PCNA, an essential gene for cell...
shRNA Mediated Target Gene Knockdown and Stable Cell Line Generation
Constitutive and Inducible shRNA Knockdown Constitutive target gene knockdownTet-inducible target gene knockdownLentivirus packaging, infection, and stable cell line generation Verify Inducible Target KD in Stable Cell Lines by RT-Taqman Verify Target KD in Stable...