B-cell-Cloning.pdf

ChemPartner expanded our antibody discovery service offering to include b cell cloning in January 2019. The Beacon^® platform is capable of screening thousands of plasma b cells from immunized animals, which speeds up a traditionally time-consuming hybridoma process.

Plasma b cells secreting antibodies of interest are selected based on cell- or bead-based binding and/or functional assays that are run serially or by multiplex format. Individual plasma b cells of interest are selectively exported for antibody sequencing. Using this platform, plasma b cells can be characterized in less than eight hours, thus accelerating the antibody discovery process.

B Cell Cloning on the Beacon^® Platform

• Reduce antibody screening time from 8-12 weeks to one day while interrogating more of the immune repertoire

• Potential to immunize WT or transgenic strains of mice

• Identify plasma B cells from immunized mice with target specific binding and functional activity

• Demonstrated functional blocking of receptor/ligand interactions using PD-L1/PD-1 as test case

• Additional assays such as Ca Flux, cytokine secretion and T cell activation are under development

• Good rate of recovery of single VH/VL sequences from successfully exported plasma b cells

Beacon^® Platform

OptoSelect™ Chip

Light manipulation + microfluidics

Single Cell Isolation in NanoPens™

Thousands in parallel

Single b cells are positioned on a chip housing thousands of sub-nL chambers called NanoPens using controlled patterns of light. Tens of thousands of single B cells can be isolated, cultured, and assayed for target specific binding and function

Typical Output for B Cell Cloning Workflow

Meet the Beacon^® Team >>

See our Recent Poster

Rapid discovery of CD28-specific antibodies from rabbits through single B cell cloning on the Beacon system