Immunofluorescence (IF) staining is used to detect and localize antigens (e.g., proteins) in cells or tissue sections by using of fluorescent dye-conjugated antibodies or non-antibody methods of fluorescent staining (eg. DAPI). Compared with immunohistochemistry (IHC), IF is more widely used to detect co-localization of multiple targets with different fluorescent dyes. 

 ChemPartner offers high-quality and efficient services for IF full assay development, protocol validation, single/double staining, image collection, and data analysis/interpretation.

• Equipment: IHC autostainer (Dako AS Link48), fluorescence microscope with 100x oil lens (Zeiss, AXIO Scope A1), etc.

• Imaging software: ZEN 2011 (Zeiss), Image Pro Plus, Image J, etc.

• Antibodies with established methods

Almost all bright-field IHC staining protocols can be converted to IF staining with fluorescent antibodies.

• Tissue types: FFPE and frozen samples of cells and tissue, TMA

Representative Images of IF Staining

Representative Images of IF Double Staining

Deliverable:

• Raw data together with analyzed data in MS Excel format

• Images in JPEG format

• Project update and summary in MS PowerPoint format

• Final report in MS Word format (including purpose, methods, results, and data analysis)

Sample Submission: Test samples could be submitted

• FFPE blocks of tissues or cells

• Oct-embedded samples

• Tissues and cells fixed in formalin

• Fresh frozen samples