Systemic inflammation in mice (Con A)
Con-A Induced Systemic Inflammation in Mice Animal: BALB/c mice, female, 9-11 weeks Modeling: ConA (15 MPK) injection through tail veins Reference drug: Dexamethasone Readouts: Body weight, liver/spleen weight, ALT and AST level in serum, inflammatory cytokines...
IMQ induced psoriasis in mice
Animal: BALB/c mice, female, 7.5 weeks Modeling: IMQ painted on shaved back skin daily Reference Drug: Tofacitinib, p.o., BID Readouts: Body weight, PASI score, skin thickness, pathology PASI Score Criterial: Erythema, scale, and thickness 0: no; 1: mild; 2: moderate;...
Adjuvant induced arthritis in rats (AIA)
Animal: Lewis rat, female, 180-200g Immunizations: CFA Readouts: body weight, joint swelling, clinical score, pathology, x-ray
Autoimmune Disease and Inflammation In Vivo Models
Respiratory Disease OVA-induced asthma in mice HDM-induced asthma in mice Bleomycin-induced lung fibrosis in mice Rheumatoid Arthritis Collagen-induced arthritis in rats (CIA) Adjuvant-induced arthritis in rats (AIA) Multiple Sclerosis (MS) MOG-induced EAE in mice...
Th Subsets Differentiation (Th1/2/17/Treg) Assays
Primary T Cell Differentiation Assay Species: human and mouse Type: Th1, Th2, Th17, Treg Cell Source: Naïve CD4+ T cell from hPBMC or mouse spleen tissue Th1: IFN-g+ Cells% by FACS Th17: IL-17a Conc. by ELISA Treg: CD25+Foxp3+ Cells% by FACS...
T Cells (Total, CD4+, CD8+) Activation and Proliferation Assays
T Cell Activation Assay Customized Assay Format Cell Source: PBMC, CD3+ T, CD4+ T, CD8+ T Stimulation: TA in the presence or absence of anti-CD3/28, PHA, PMA, SEB Readout: Cell proliferation (CTG/FACS), cytokine release (ELISA/Luminex) ...
Mixed Lymphocyte Reaction (MLR) Assay
The MLR assay is considered the ‘gold standard’ T cell functional assay for the test of T cell immune checkpoint inhibitors Dendritic cells co-cultured with allogeneic CD3+ T cells Readouts: IFN-γ and IL-2 production Suitable to test both antibodies and small...
Whole Blood/PBMC Stimulation and Cytokine Release Assay
LPS-induced Cytokine Release in Human PBMC