Target-Specific Assays – Antibody Targets

由 Sarah Stapleton | 3月 5, 2021

ID Target Receptor Ligand Binding Blocking Cell-based Functional Readout 1 PD1 PD1/PD-L1 PD1/PD-L2 T cell activation (PBMC + CHOK1/OS8/PD-L1) Mixed Lymphocyte Reaction Jurkat NFAT luciferase reporter assay 2 PD-L1 PD-L1/PD1 PD-L1/B7.1 T cell activation (PBMC +...

Functional Assay Panels

由 Sarah Stapleton | 3月 5, 2021

  ID Assay Type Cell Type 1 Mixed Lymphocyte Reaction (MLR) Primary human/mouse DC + T cells 2 CMV antigen recall Primary human T cells 3 T cell activation (anti-CD3/CD28) Primary human/mouse T cells 4 T cell activation (SEB super-antigen) Primary human T cells 5 T...

Luminex

由 Sarah Stapleton | 3月 5, 2021

Customized commercial kits Multiplexing with up to 80 analytes per well Validated Analytes Pool 253 Human targets 108 Mouse targets 104 Monkey targets 35 Rat targets QC Criterial Inner and inter-plate standard signal CV tracking Luminex Reader List 2D System Bio-Plex...

Multi-color Flow Cytometry

由 Sarah Stapleton | 3月 5, 2021

Multi-channel flow cytometer Customized panel design 96/384-well plate auto-sampler High-throughput screening Method development service     Thermo Attune NxT   Gentle MACS Dissociator   Beckman...

Ex Vivo Analysis Platforms

由 cstolzerhy | 3月 5, 2021

Multi-color Flow Cytometry Luminex Quantigene ELISPOT

Systemic inflammation in mice (Con A)

由 Sarah Stapleton | 3月 5, 2021

Con-A Induced Systemic Inflammation in Mice Animal: BALB/c mice, female, 9-11 weeks Modeling: ConA (15 MPK) injection through tail veins Reference drug: Dexamethasone Readouts: Body weight, liver/spleen weight, ALT and AST level in serum, inflammatory cytokines...

IMQ induced psoriasis in mice

由 Sarah Stapleton | 3月 5, 2021

Animal: BALB/c mice, female, 7.5 weeks Modeling: IMQ painted on shaved back skin daily Reference Drug: Tofacitinib, p.o., BID Readouts: Body weight, PASI score, skin thickness, pathology PASI Score Criterial: Erythema, scale, and thickness 0: no; 1: mild; 2: moderate;...

Adjuvant induced arthritis in rats (AIA)

由 Sarah Stapleton | 3月 5, 2021

Animal: Lewis rat, female, 180-200g Immunizations: CFA Readouts: body weight, joint swelling, clinical score, pathology, x-ray    

Autoimmune Disease and Inflammation In Vivo Models

由 cstolzerhy | 3月 5, 2021

Respiratory Disease OVA-induced asthma in mice HDM-induced asthma in mice Bleomycin-induced lung fibrosis in mice Rheumatoid Arthritis Collagen-induced arthritis in rats (CIA) Adjuvant-induced arthritis in rats (AIA) Multiple Sclerosis (MS) MOG-induced EAE in mice...

Th Subsets Differentiation (Th1/2/17/Treg) Assays

由 Sarah Stapleton | 3月 5, 2021

Primary T Cell Differentiation Assay Species: human and mouse Type: Th1, Th2, Th17, Treg Cell Source: Naïve CD4+ T cell from hPBMC or mouse spleen tissue   Th1: IFN-g+ Cells% by FACS   Th17: IL-17a Conc. by ELISA   Treg: CD25+Foxp3+ Cells% by FACS...