Mixed Lymphocyte Reaction (MLR) Assay
The MLR assay is considered the ‘gold standard’ T cell functional assay for the test of T cell immune checkpoint inhibitors Dendritic cells co-cultured with allogeneic CD3+ T cells Readouts: IFN-γ and IL-2 production Suitable to test both antibodies and small...
Whole Blood/PBMC Stimulation and Cytokine Release Assay
LPS-induced Cytokine Release in Human PBMC
Primary Immune Cell In Vitro Assays
Whole Blood/PBMC Stimulation and Cytokine Release Assay Mixed Lymphocyte Reaction (MLR) Assay CMV Antigen Recall Assay Tumor/T Cell Co-culture Assay T Cells (Total, CD4+, CD8+) Activation and Proliferation Assays Th Subsets Differentiation (Th1/2/17/Treg) Assays Treg...
Case Studies in the Syngeneic Model
Efficacy Test in CT2.WT Model Tumor Growth Survival Evaluation (Endpoint: TV > 2000mm3 Mice under combination therapy exhibited survival benefit Efficacy Test in 4T1 Resection Model Tumors were resected on D13; Half mice to observe survival;...
Case Studies in PDXs
Mouse Clinical Trial in PDXs Objective: to evaluate drug efficacy across 17 colorectal PDXs Pre-defined Clinical Endpoints Tumor Progression A tumor volume change >73% by the end of treatement Tumor Stasis A tumor volume change between -66% and 73% by the end of...
Model Generation Study in CDXs
Tumor Volume Change of NCl-H661 subQ Model Total Flux in U87MG-Luc2 Orthotopic Model
Case Studies in CDXs
Efficacy Study in CDXs Tumor Growth Inhibition in MV-4-11 Survival Rate in A375 Brain Inoculation Tumor Growth Inhibition in Hep3B 2.1-7 Efficacy and Biomarker Assessment in CDXs Cancer Discovery. 2018 Jul;8(7):836-849 Cell line derived allograft and xenograft models...
免疫荧光 (IF)
Immunofluorescence (IF) staining is used to detect and localize antigens (e.g., proteins) in cells or tissue sections by using of fluorescent dye-conjugated antibodies or non-antibody methods of fluorescent staining (eg. DAPI). Compared with immunohistochemistry...