|
ID |
Target |
Biochemical Assay |
Cell-based Assays |
|
1 |
IDO1 |
IDO1 enzyme activity |
IFNγ-induced cellular IDO1 in Hela cells; IDO1 transient/stable transfection in HEK293 cells |
|
2 |
TDO2 |
TDO2 enzyme activity |
TDO2 transient/stable transfection in HEK293 cells |
|
3 |
ARG1 |
ARG1 enzyme activity |
NA |
|
4 |
A2A |
A2A binding activity |
Cellular cAMP; NECA-mediated T cell functional (TNFα and p-CREB level) |
|
5 |
A2B |
NA |
Cellular AMP |
|
6 |
STING |
STING thermal shift assay |
STING/TBK1/IRF3 pathway by Western blot |
|
7 |
ALK4 |
ALK4 Kinase activity |
NA |
|
8 |
ALK5 |
ALK5 Kinase activity |
TGFβ1-induced SBE (SMAD binding element) reporter activity |
|
9 |
PI3Kγ |
PI3Kγ Kinase activity |
p-AKT by ELISA |
|
10 |
PI3Kδ |
PI3Kδ Kinase activity |
p-AKT by ELISA |
