Immunogen and Antigen Preparation
- Recombinant protein production and cell line development
- Peptide and compound design and synthesis
- DNA In vivo transfection
Binding Assays and Bioassays
- Well-established assays
- Assay development and optimization for hybridoma screening
- Extensive functional assays for antibody characterization
| Item | Well Established Assays |
| Protein-based Functional Assays |
Ligand binding/blocking assay (Protein based ELISA or cell based FACS) |
| Cell based or protein based enzymatic assays (such as CD38, CD39, CD73, complement pathway…) | |
| Cell-based Functional Assays
30 + Established assays Recombinant cell lines Endogenous cell lines Primary human PBMC Primary NHP cells Primary rodent cells |
Ligand binding/blocking assay(FACS) |
| Proliferation assay | |
| Apoptosis assay | |
| Mixed Lymphocyte Reaction Assay | |
| T Cell Stimulation Assay | |
| Cell adhesion blocking assay | |
| Migration assay | |
| Soft agar colony formation assay | |
| Phosphorylation/signaling assay | |
| Luciferase reporter assay | |
| Cytokine releasing assay | |
| FLIPR assay | |
| Antibody internalization assay | |
| ADCC/CDC | |
| Whole blood assay | |
| Lymphocytes/tumor cell co-culture killing assay |
AAALAC accredited and licensed animal facility
Animal Immunization
- Multiple Specific Pathogen Free (SPF) species: mouse, rat, hamster, rabbit
- CPSJ mice with superior immune response
- Transgenic mice for fully human antibody generation
- Customized program based on target biology and assays
Hybridoma Development
- High fusion efficiency electrofusion
- ELISA/Acumen/Mirrorball screening and customized functional screening
- Isotyping/epitope binning by ELISA/Octet and affinity ranking by Biacore/Octet
- High throughput SPR/Epitope binning by LSA Carterra
Antibody Production
- Working and master cell banking
- In vitro production and affinity purification
- Low endotoxin purification for bioassay and in vivo studies
- Rigorous quality and functional characterization
