by Sarah Stapleton Mar 5, 2021
TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...
by Sarah Stapleton Mar 5, 2021
Readouts Frequency of immune cell subpopulation Expression of immune checkpoints and markers Soluble factors Receptor Occupancy Methods Flow cytometry IHC, IF ELISA, Luminex, CBA ELISpot Western blot, qPCR Tissues Tumor Spleen Lymph nodes Peripheral blood...
by Sarah Stapleton Mar 5, 2021
MOA/Biomarker Cmpd X currently in clinical trials.
by Sarah Stapleton Mar 5, 2021
ID Target Biochemical Assay Cell-based Assays 1 IDO1 IDO1 enzyme activity IFNγ-induced cellular IDO1 in Hela cells; IDO1 transient/stable transfection in HEK293 cells 2 TDO2 TDO2 enzyme activity TDO2 transient/stable transfection in HEK293 cells 3 ARG1 ARG1 enzyme...
by Sarah Stapleton Mar 5, 2021
ID Target Receptor Ligand Binding Blocking Cell-based Functional Readout 1 PD1 PD1/PD-L1 PD1/PD-L2 T cell activation (PBMC + CHOK1/OS8/PD-L1) Mixed Lymphocyte Reaction Jurkat NFAT luciferase reporter assay 2 PD-L1 PD-L1/PD1 PD-L1/B7.1 T cell activation (PBMC +...
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