Bay Area Peptalks
- Manuel Sanchez-Felix, PhD, Senior Fellow, Novel Delivery Technologies Group at Novartis Institutes for BioMedical Research
- Robert Tarran, PhD, President and CSO, Eldec Pharmaceuticals and Professor of Cell Biology & Physiology at the University of North Carolina at Chapel Hill (UNC)
Patient-centric design for systemic peptide delivery: Trends in routes of administration and advancement in drug delivery technologies
Utilizing peptides as therapeutic agents is considered an attractive approach for treating various diseases due to their high binding and selectivity to novel drug targets. However, the physicochemical properties of peptides make them challenging to administer exogenously, which places a high burden on drug delivery technology selection to successfully achieve the desired response. Various peptide drug delivery systems have been used in clinical and marketed products to overcome the problems associated with exogenous administration of peptide therapeutics. This presentation covers trends observed pertaining to the delivery of peptides such as changes in the routes of administration, advancements in formulation platforms, patient-centric product design, and various other aspects of peptide delivery.
Harnessing the Lung’s Naturally Occurring Peptidome to Generate Novel Therapeutics
Airway secretions contain ~1000 proteins and peptides that play important roles in regulating lung homeostasis. In many lung diseases including cystic fibrosis (CF), chronic neutrophilia and infection lead to an increase in free protease levels that induce protein/peptide cleavage, suggesting an impaired/altered functionality. Little is known about the peptidome of airway secretions. Here, we set out to define the normal and CF peptidomes and to use them as a drug library for future studies. We size-fractionated sputum from normal and CF subjects and studied these fractions by LC-MS/MS without conventional tryptic digestion in order to detect endogenously produced peptides. We identified ~130 endogenous peptides in normal sputum and ~700 peptides that were common to all CF patients. Interestingly, only 60 peptides were seen in both normal and CF subjects. Using principle component analysis and k-means clustering, we found that the normal and CF peptidomes were p<0.00013 different. Since little is known about the functionality of the normal and CF peptidomes, we utilized SPOT synthesis technology to make a library of these peptides that included the entire normal and CF peptidomes. We then performed high content imaging using 384 well plates in order to determine whether these peptides could affect cell growth, cell Ca2+ and some other parameters including viral infection with respiratory syncytial virus and a pseudotyped SARS-CoV-2 S1 virus. Using this approach, we identified peptides that inhibited Ca2+ signaling, which Eldec is now developing as novel immunomodulatory peptides for lung diseases including pneumonia, asthma and fibrosis, and also identified novel antiviral peptides. These data indicate that peptidome screening is a feasible starting point for developing novel peptide-based therapeutics. — Funded by the NIH and North Carolina Biotechnology Center.
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Application of In-Vitro and In-Vivo ADME/PK/Exploratory Tox Assays to Support Lead and Candidate Selections
Presented by Dr. Lilly Xu, Ph.D., Senior Vice President, DMPK & Exploratory Toxicology at ChemPartner
Topics Discussed in this Webinar are:
- In-vitro ADME and tox assays
- In-vivo PK/Expl Tox assays
- Application of these assays for lead and candidate selections
- IND filing package summary
The History of Cancer Drugs
Presented by Dr. Jie Jack Li, Vice President of Discovery Chemistry at ChemPartner
Anticancer drugs have experienced a revolution during the last decades with the emergence of kinase inhibitors as targeted drugs and immune-oncology. This webinar covers a history of small molecule anticancer drugs from the perspective of a medicinal chemist. It starts with a brief discussion of the etiology of cancer and then moves on to review eight classes of cancer drugs. All scientists in oncology, especially medicinal chemists, will find this webinar informational and entertaining.
COVID-19 Drugs: Discovery, Mechanism of Action, and Synthesis
Presented by Dr. Jie Jack Li, Vice President of Discovery Chemistry at ChemPartner
COVID-19, an infectious disease caused by SARS-CoV-2, is now wreaking havoc in the US and around the world. Meanwhile, scientists are also making strides against the invisible enemy. Most notable are the recent success of remdesivir and demethasone.
This webinar, given from a chemist’s perspective, covers the discovery, mechanism of action, and synthesis of remdesivir and demethasone. It is most relevant to medicinal and process chemists. But anyone interested in drug discovery will find it highly informational as well.
Drugging the Undruggable Cancer Targets
Presented by Dr. Ming Liu, Senior Vice President of Biology and Pharmacology at ChemPartner
Cancer is a multifactorial disease, and its genesis and progression are extremely complex. The scope of precision oncology is rapidly expanding to address previously undruggable targets.
The term “undruggable” was used to describe proteins that could not be targeted pharmacologically. Many targets in cancer research fall into this category, including KRAS, which has eluded researchers for more than 30 years until recent landmark discoveries of AMG 510, MRTX 849, and BI 1701963. As clinical trials are vigorously advanced, efficacy, safety, resistance, and combination strategies are being carefully evaluated for those drug candidates. In addition to KRAS, there are other challenging targets such as P53, MYC, fusion transcription factors, immuno-suppressors,…etc.
The focus of this webinar is to provide an overview of strategies in drugging some perceived undruggable targets, with stories and case studies by scientists who strive to find these medicines and fill unmet patient needs.
Experienced in overcoming prior hurdles to find effective antagonists against cancer, as a R&D CRO, ChemPartner has utilized a wide range of integrated approaches such as medicinal chemistry, antibody-drug conjugates, monoclonal antibodies, in vitro biology assays, cell biology, immuno-oncology, pharmacology, and combination therapy to identify and tackle the problems that surround drug development for “undruggable” targets.
Drug Discovery Advanced with Modern Externalized Models
Presented by Dr. Sarah Lively, Vice President of Innovation and New Technology at ChemPartner
The world of external support to advance drug discovery has changed dramatically in the last 20 years. We are now in a position where we can truly rely on external partners to ‘get the job done’ and can run our programs almost entirely virtually from early target validation all the way through IND enabling studies, FIH, and beyond. This talk will explain some of the newer working models of support and the innovative ways service providers are finding to support their customers. In particular, it will focus on the science around select case studies in oncology and neuroscience where ChemPartner has partnered with groups to push projects efficiently to a key inflection point.
These case studies will showcase the key decision-making criteria biopharmaceutical companies use to determine their ‘partner of choice.’ Additionally, we’ll review the kinds of high-end science that pharma and biotech companies can now find outside of their own walls and how groups can come together to achieve common goals. Lastly, we’ll discuss ways to form strategic partnerships with different groups in a real ‘win-win’ scenario where all partners participate in the upsides of success. This goes beyond traditional ‘risk share’ which are more ‘risk mitigation’ strategies on the part of groups looking to parse off risk where outcomes are less certain, but more where savvy groups come together for mutual benefit and true and lasting partnerships form.
Rapid Discovery of Potent anti-PD-L1 Antibodies Enabled by Single Plasma B Cell Cloning on the Beacon Platform and Epitope Binning with the Carterra LSA
Presented by Dr. Shrieen Khan, Senior Director of Biologics at ChemPartner
- Antibody discovery in weeks versus years
- Isolate, culture, and assay tens of thousands of single cells with the Beacon platform
- Survey the epitope landscape of your antibody panel at the earliest stages of discovery with the Carterra LSA
PD-L1 is a key inhibitor of T cell activation that is often over-expressed in cancer to escape immune surveillance and promote tumor progression. Blocking antibodies against PD-L1 or its receptor, PD-1, have shown significant clinical benefit in some patients with PD-L1 expressing tumors. Hence, there is great interest in generating therapeutic antibodies against these targets to counteract the immune suppression mechanism that tumors rely on for survival.
Epitope binning studies on the Carterra LSA revealed that hybridoma antibodies covered more diverse epitope regions but potency in functional assays was weaker across all bins while B Cell Cloning antibodies were concentrated in fewer epitope bins that represented greater potency.
Our data show that plasma B cells secreting functional antibody candidates can be rapidly identified on the Beacon compared to several months for a hybridoma campaign and screening those antibodies for epitope specificity and affinity can be completed in just days on the Carterra LSA, thus substantially accelerating the antibody discovery process.
Rapid Discovery of a Diverse Panel of Antibodies Against Membrane Targets Using the Beacon System
Presented by Dr. Shireen Khan, Senior Director of Biologics at ChemPartner and Dina Sirypangno, Senior Technical Sales Specialist at Berkeley Lights
Traditional antibody discovery technologies have been used to develop all FDA-approved antibody therapeutics currently in the clinic for diseases including autoimmune, inflammatory, infectious diseases, and cancer. However, the next-generation of antibody therapies against more challenging targets, such as membrane-bound receptors, will require new technologies that can screen the broad B cell repertoire in depth.
Dr. Shireen Khan presents a case study of how ChemPartner has leveraged the Beacon system to rapidly discover cross-reactive antibodies against the GPI-anchored urokinase plasminogen activator (uPA) receptor. uPA and its receptor uPAR are associated with aggressive forms of cancer including metastatic breast cancer and pancreatic ductal adenocarcinomas. The upregulation of uPA in cancer was shown to correlate with poor clinical prognosis and supports targeting this pathway as a therapeutic approach for tumors in which uPA/uPAR are tumorigenic drivers. Identification of crossreactive antibodies to human, cyno, and mouse uPAR proved to be challenging by panning a Fab phage display library against human uPAR.
In collaboration with UCSF and SPII, ChemPartner leveraged the Berkeley Lights’ Beacon optofluidic system to screen deeper into the immune B cell repertoire. Single B cells were screened on the Beacon system using a novel multiplexed assay for human, mouse, and cyno uPAR binders. Antibodies were identified that bound both human and cyno uPAR proteins and represented broad sequence diversity. After generating the antibodies through recombinant antibody expression and purification, several candidates were strong binders to human uPAR on endogenously expressing MDA-MB-231 breast cancer cells. Functional characterization of these antibodies in uPA/uPAR blocking, pERK signaling, and invasion assays is underway.
Dina Sirypangno shares new capabilities developed at Berkeley Lights to enable better antibody lead candidate selection in under 1 week.