Classical sandwich assay format was selected for epitope binning using Octet Red 384. About nine bins were generated based on the result. Classical Sandwich Assay Sandwich format requirement: Ag reach equilibrium in 5 minutes Disassociation of Ag <50% in 10 minutes...
KD measurement of peptide binding to target protein: kinetics, affinity. In the below case, more than 500 peptides were tested and the KD of which range from mM to nM. Positive Peptide Peptide A Peptide B
Immobilization Anti-hFc Capture Protein A Capture Protein G Capture High quality KD data can be obtained by various formats, such as: Anti-His Capture Anti-mFc Capture SA
Kinetics | Affinity | Thermodynamics How fast, strong & why… Is the binding of a lead antibody or compound? Specificity How specific & selective… Is this drug binding to its receptor? Concentration How much… Is the biologically active component in...
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