by Corah Whipple | Mar 4, 2021
We offer multiple assay formats targeting the mAb portion, toxin, and the intact ADC drug. Single Intravenous Pharmacokinetics Study of Antibody Drug Conjugate (#1015) in Cynomolgus Monkey by Using Kadcyla® as ReferenceTarget Mediated ADC Clearance in Cynomolgus...
by Corah Whipple | Mar 3, 2021
Classical sandwich assay format was selected for epitope binning using Octet Red 384. About nine bins were generated based on the result. Classical Sandwich Assay Sandwich format requirement: Ag reach equilibrium in 5 minutes Disassociation of Ag <50% in 10 minutes...
by Corah Whipple | Mar 3, 2021
KD measurement of peptide binding to target protein: kinetics, affinity. In the below case, more than 500 peptides were tested and the KD of which range from mM to nM. Positive Peptide Peptide A Peptide B
by Corah Whipple | Mar 3, 2021
Immobilization Anti-hFc Capture Protein A Capture Protein G Capture High quality KD data can be obtained by various formats, such as: Anti-His Capture Anti-mFc Capture SA
by cstolzerhy | Mar 3, 2021
Kinetics | Affinity | Thermodynamics How fast, strong & why… Is the binding of a lead antibody or compound? Specificity How specific & selective… Is this drug binding to its receptor? Concentration How much… Is the biologically active component in...
by Corah Whipple | Mar 3, 2021
Potential CDR hot spots analysis Biophysical characterization Short-term stability studies Limited forced degradation studies High concentration possibility assessment
by Corah Whipple | Mar 3, 2021
Mouse, rat, monkey SC, IP, IV bolus, IV infusion Bio-analytical development PK/PD modeling and simulation In vitro stability in biological matrices Single dose, repeated dose, dose range finding in rats and monkeys Efficacy studies 160+ cell line xenografts and 270+...
by cstolzerhy | Mar 3, 2021
Our capabilities include the following assays: Antigen binding activity determination: ELISA, FACS Drug to Antibody ratio (DAR) determination: UV-Vis, LC-MS, HPLC-HIC, iCIEF, etc. Residues of free drug determination: LC-MS/MS In vitro efficacy and bioactivity...
by Corah Whipple | Mar 3, 2021
Concentration determination: spectrophotometry Endotoxin level determination: LAL method Drug to antibody ratio (DAR) determination: LC-MS, HPLC-HIC, RP-HPLC, etc. Purity determination: SEC/ RP/ HIC-HPLC, SDS-PAGE, etc. Residues of free drug determination: LC-MS/MS...
