Behavioral Pharmacology

by Corah Whipple | Mar 5, 2021

Neurodegeneration Alzheimer’s Disease – 5x Familial AD Transgenic Mice Biomarkers, pathology, EEG, behavioral tests, LTP in brain slice Parkinson’s Disease – 6-OHDA Rats Apomorphine or L-DOPA challenged rotation L-DOPA induced dyskinesia in 6-OHDA rats...

In Vitro Electrophysiology and Ex Vivo Assays

by cstolzerhy | Mar 5, 2021

Ion Channel Targets Voltage-gated Ion Channels (K+) hERG (Na+) Nav 1.5, Nav 1.7, Nav 1.1 Ligand-gated Ion Channels TRP Family (TRPV1, V3, C6) P2X Family (P2X3, 4, 7) GABAaR (1α, 2β, γ2) GluR (AMPA, NMDA) Family In Vitro and Ex Vivo CNS Biomarkers High content image...

TIL Subpopulation Screening in Syngeneic Models

by Sarah Stapleton | Mar 5, 2021

TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca         Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...

Readouts and Methods – Ex Vivo Analysis

by Sarah Stapleton | Mar 5, 2021

Readouts Frequency of immune cell subpopulation Expression of immune checkpoints and markers Soluble factors  Receptor Occupancy     Methods Flow cytometry IHC, IF ELISA, Luminex, CBA ELISpot Western blot, qPCR     Tissues Tumor  Spleen Lymph nodes Peripheral blood...

Case Study: Driving to Clinical Development

by Sarah Stapleton | Mar 5, 2021

MOA/Biomarker Cmpd X currently in clinical trials.

Target-Specific Assay Panels – Small Molecule Targets

by Sarah Stapleton | Mar 5, 2021

ID Target Biochemical Assay Cell-based Assays 1 IDO1 IDO1 enzyme activity IFNγ-induced cellular IDO1 in Hela cells;  IDO1 transient/stable transfection in HEK293 cells 2 TDO2 TDO2 enzyme activity TDO2 transient/stable transfection in HEK293 cells 3 ARG1 ARG1 enzyme...

Target-Specific Assays – Antibody Targets

by Sarah Stapleton | Mar 5, 2021

ID Target Receptor Ligand Binding Blocking Cell-based Functional Readout 1 PD1 PD1/PD-L1 PD1/PD-L2 T cell activation (PBMC + CHOK1/OS8/PD-L1) Mixed Lymphocyte Reaction Jurkat NFAT luciferase reporter assay 2 PD-L1 PD-L1/PD1 PD-L1/B7.1 T cell activation (PBMC +...

Functional Assay Panels

by Sarah Stapleton | Mar 5, 2021

  ID Assay Type Cell Type 1 Mixed Lymphocyte Reaction (MLR) Primary human/mouse DC + T cells 2 CMV antigen recall Primary human T cells 3 T cell activation (anti-CD3/CD28) Primary human/mouse T cells 4 T cell activation (SEB super-antigen) Primary human T cells 5 T...

Luminex

by Sarah Stapleton | Mar 5, 2021

Customized commercial kits Multiplexing with up to 80 analytes per well Validated Analytes Pool 253 Human targets 108 Mouse targets 104 Monkey targets 35 Rat targets QC Criterial Inner and inter-plate standard signal CV tracking Luminex Reader List 2D System Bio-Plex...

Multi-color Flow Cytometry

by Sarah Stapleton | Mar 5, 2021

Multi-channel flow cytometer Customized panel design 96/384-well plate auto-sampler High-throughput screening Method development service     Thermo Attune NxT   Gentle MACS Dissociator   Beckman...