In Vitro Electrophysiology and Ex Vivo Assays
Ion Channel Targets Voltage-gated Ion Channels (K+) hERG (Na+) Nav 1.5, Nav 1.7, Nav 1.1 Ligand-gated Ion Channels TRP Family (TRPV1, V3, C6) P2X Family (P2X3, 4, 7) GABAaR (1α, 2β, γ2) GluR (AMPA, NMDA) Family In Vitro and Ex Vivo CNS Biomarkers High content image...
TIL Subpopulation Screening in Syngeneic Models
TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...
Readouts and Methods – Ex Vivo Analysis
Readouts Frequency of immune cell subpopulation Expression of immune checkpoints and markers Soluble factors Receptor Occupancy Methods Flow cytometry IHC, IF ELISA, Luminex, CBA ELISpot Western blot, qPCR Tissues Tumor Spleen Lymph nodes Peripheral blood...
Case Study: Driving to Clinical Development
MOA/Biomarker Cmpd X currently in clinical trials.
Target-Specific Assay Panels – Small Molecule Targets
ID Target Biochemical Assay Cell-based Assays 1 IDO1 IDO1 enzyme activity IFNγ-induced cellular IDO1 in Hela cells; IDO1 transient/stable transfection in HEK293 cells 2 TDO2 TDO2 enzyme activity TDO2 transient/stable transfection in HEK293 cells 3 ARG1 ARG1 enzyme...
Target-Specific Assays – Antibody Targets
ID Target Receptor Ligand Binding Blocking Cell-based Functional Readout 1 PD1 PD1/PD-L1 PD1/PD-L2 T cell activation (PBMC + CHOK1/OS8/PD-L1) Mixed Lymphocyte Reaction Jurkat NFAT luciferase reporter assay 2 PD-L1 PD-L1/PD1 PD-L1/B7.1 T cell activation (PBMC +...
Functional Assay Panels
ID Assay Type Cell Type 1 Mixed Lymphocyte Reaction (MLR) Primary human/mouse DC + T cells 2 CMV antigen recall Primary human T cells 3 T cell activation (anti-CD3/CD28) Primary human/mouse T cells 4 T cell activation (SEB super-antigen) Primary human T cells 5 T...
Luminex
Customized commercial kits Multiplexing with up to 80 analytes per well Validated Analytes Pool 253 Human targets 108 Mouse targets 104 Monkey targets 35 Rat targets QC Criterial Inner and inter-plate standard signal CV tracking Luminex Reader List 2D System Bio-Plex...