CRISPR/Cas9 Mediated Target Gene Knockout
Validation of Cas9 Expression by Western Blot Validation of Cas9 Expression by FACS Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection) CRISPR Target Gene Knockout – Phenotypic Study Knockout of PCNA, an essential gene for cell...
shRNA Mediated Target Gene Knockdown and Stable Cell Line Generation
Constitutive and Inducible shRNA Knockdown Constitutive target gene knockdownTet-inducible target gene knockdownLentivirus packaging, infection, and stable cell line generation Verify Inducible Target KD in Stable Cell Lines by RT-Taqman Verify Target KD in Stable...
siRNA Mediated Target Gene Knockdown
Knock down target gene by siRNA pool or individual oligos Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay...
Behavioral Pharmacology
Neurodegeneration Alzheimer’s Disease – 5x Familial AD Transgenic Mice Biomarkers, pathology, EEG, behavioral tests, LTP in brain slice Parkinson’s Disease – 6-OHDA Rats Apomorphine or L-DOPA challenged rotation L-DOPA induced dyskinesia in 6-OHDA rats...
In Vitro Electrophysiology and Ex Vivo Assays
Ion Channel Targets Voltage-gated Ion Channels (K+) hERG (Na+) Nav 1.5, Nav 1.7, Nav 1.1 Ligand-gated Ion Channels TRP Family (TRPV1, V3, C6) P2X Family (P2X3, 4, 7) GABAaR (1α, 2β, γ2) GluR (AMPA, NMDA) Family In Vitro and Ex Vivo CNS Biomarkers High content image...
TIL Subpopulation Screening in Syngeneic Models
TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...
Readouts and Methods – Ex Vivo Analysis
Readouts Frequency of immune cell subpopulation Expression of immune checkpoints and markers Soluble factors Receptor Occupancy Methods Flow cytometry IHC, IF ELISA, Luminex, CBA ELISpot Western blot, qPCR Tissues Tumor Spleen Lymph nodes Peripheral blood...
Case Study: Driving to Clinical Development
MOA/Biomarker Cmpd X currently in clinical trials.