Validation of Cas9 Expression by Western Blot Validation of Cas9 Expression by FACS Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection) CRISPR Target Gene Knockout – Phenotypic Study Knockout of PCNA, an essential gene for cell...
Knock down target gene by siRNA pool or individual oligos Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay...
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