siRNA Mediated Target Gene Knockdown


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Knock down target gene by siRNA pool or individual oligos

  • Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively
  • The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay (2D) and/or soft agar colony formation assay (3D)


Taqman KD Evaluation


Western Blot KD Evaluation (GAPDH as an example)


siRNA Anti-Proliferation CTG Assay in Cell Line X


siRNA Soft Agar Screen in Cell Line X


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