Validation of Cas9 Expression by Western Blot Validation of Cas9 Expression by FACS Detect Target B Expression by Western Blot in Single KO Clones (Transient Transfection) CRISPR Target Gene Knockout – Phenotypic Study Knockout of PCNA, an essential gene for cell...
Knock down target gene by siRNA pool or individual oligos Taqman or Western blot analysis to confirm the knockdown efficiency at mRNA level or protein level, respectively The effect of tumor cell growth inhibition is monitored by CellTiter Glo cell viability assay...
Ion Channel Targets Voltage-gated Ion Channels (K+) hERG (Na+) Nav 1.5, Nav 1.7, Nav 1.1 Ligand-gated Ion Channels TRP Family (TRPV1, V3, C6) P2X Family (P2X3, 4, 7) GABAaR (1α, 2β, γ2) GluR (AMPA, NMDA) Family In Vitro and Ex Vivo CNS Biomarkers High content image...
TIL baselines were profiled in top ten popular tumor models: CT26.WT MC-38 4T1 B16-F10 EL4 EMT-6 Hepa1-6 A20 LLC1 Renca Data expressed are average values of 3 tumors of 500-1000 mm3 for each model. ...
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