Th Subsets Differentiation (Th1/2/17/Treg) Assays


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Primary T Cell Differentiation Assay

  • Species: human and mouse
  • Type: Th1, Th2, Th17, Treg
  • Cell Source: Naïve CD4+ T cell from hPBMC or mouse spleen tissue


Th1: IFN-g+ Cells% by FACS


Th17: IL-17a Conc. by ELISA


Treg: CD25+Foxp3+ Cells% by FACS


Primary T Cell Differentiation Assay: Treg Data

  • Human or mouse naïve CD4+ T cells induced by anti-CD3, IL-2 and TGFβ 
  • Treg cells identified by FoxP3+ or FoxP3+CD25+ population


Human Naïve CD4+ T Cells


Mouse Naïve CD4+ T Cells



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