KD measurement of peptide binding to target protein: kinetics, affinity. In the below case, more than 500 peptides were tested and the KD of which range from mM to nM. Positive Peptide Peptide A Peptide B
Immobilization Anti-hFc Capture Protein A Capture Protein G Capture High quality KD data can be obtained by various formats, such as: Anti-His Capture Anti-mFc Capture SA
Kinetics | Affinity | Thermodynamics How fast, strong & why… Is the binding of a lead antibody or compound? Specificity How specific & selective… Is this drug binding to its receptor? Concentration How much… Is the biologically active component in...
Mouse, rat, monkey SC, IP, IV bolus, IV infusion Bio-analytical development PK/PD modeling and simulation In vitro stability in biological matrices Single dose, repeated dose, dose range finding in rats and monkeys Efficacy studies 160+ cell line xenografts and 270+...
Our capabilities include the following assays: Antigen binding activity determination: ELISA, FACS Drug to Antibody ratio (DAR) determination: UV-Vis, LC-MS, HPLC-HIC, iCIEF, etc. Residues of free drug determination: LC-MS/MS In vitro efficacy and bioactivity...
Concentration determination: spectrophotometry Endotoxin level determination: LAL method Drug to antibody ratio (DAR) determination: LC-MS, HPLC-HIC, RP-HPLC, etc. Purity determination: SEC/ RP/ HIC-HPLC, SDS-PAGE, etc. Residues of free drug determination: LC-MS/MS...
Trastuzumab-DM1 (T-DM1; biosimilar of Kadcyla®) was synthesized and was tested in an in vitro efficacy assay employing an in vivo xenograft model. The following results demonstrate the in-house T-DM1 has comparable tumor inhibition activity and in vivo PK profiles...
Apart from antibody humanization and antibody discovery from phage display, ChemPartner’s antibody engineering department also offers sequence optimization and affinity maturation services. We provide customized affinity maturation strategies [random mutagenesis...
ChemPartner’s proprietary fully human naïve phage library with diversity ~10^11. Efficient library selection and screening with high-throughput robotics. All V-genes were amplified with high fidelity from diverse healthy donors. Stringent cloning procedures ensure a...
