A total of 33,377 plasma cells were screened from PD-L1-immunized mice and the secreted antibodies were tested for binders to PD-L1 and blockers of the interaction between PD-L1 and PD-1. The screening assays were completed and a total of 35 antibodies (BCC-Abs) that bound PD-L1 and blocked the interaction with PD-1 were identified on the day the mice were sacrificed. Single cell sequencing allowed the team to recover 24 paired VH/VL sequences, of which 23 could be expressed and purified.
I have established many different kinds of cell-based assay platforms and was responsible for. the set up of the CRISPR based library screening platform.
With help from my supervisors, I have been trained from a senior scientist to a principal scientist. And after working on many diverse projects with different team members, I am responsible for the training, supervising & managing of the team.
There are so many new targets, new platforms, and new technologies.
I am learning to cook from the experts in Bilibili.
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