- Cell culture process technology platform provides for the optimization of media, seeding densities, feeding and bioreactor process control strategies to maximize expression, and enhances process reproducibility and facilitate scale-up
- Adaption of cell line to suspension culture process
- Adaption of cell line to serum-free/animal protein-free media
- Media optimization and evaluation
- Development of fed-batch bioreactor process
- Cell culture characterization and optimization
- Scale-up
- Analysis of risk and cost benefits for various types of cell culture processes
Cell Culture Systems
- Flasks (all types)
- Spinner/shake flasks
- Wave- and stir-form bioreactors
- 3L and 15L bioreactors (Applikon)
- Wave-form Bioreactors
- 2L – 25L capacity (GE)
- Single-use bioreactor (Hyclone & GE)
- 1 x 50L
- 1x200L
- 1x250L
- Cell culture analyzers
- Bioprofile Flex2 (Nova Biomedical)
- Bioprofile 400 (Nova Biomedical)
- Vi-cell (Beckman Coulter)
- Cell culture clarification
- Depth filtration
- Centrifugation
Downstream Process Platform
- Affinity chromatography
- Cation or Anion exchange chromatography
- Hydrophobic chromatography
- Mixed mode chromatography
- Depth filtration system
- 0.22 μm filtration system
- Nanofiltration
- Ultra-filtration and diafiltration
- Virus inactivation system
- Membrane chromatography
- Periodic counter-current chromatography (PCC)
- Akta Avant, Akta Pure, Crossflow
- Akta Pilot
- Akta Ready, Akta Process
- Automatic UFDF system (Pall, Merck)
- Scale up tool and tech transfer tools
Chromatography
Normal Flow Filtration: Vmax and Pmax
TFF: Cross-flow
