- Specific Pathogen Free (SPF) animals and multiple species/strains such as SJL mice and SD rats
- New Zealand White rabbit are available for immune phage library approach
- Animal strains with superior immune response to highly conserved and multi-transmembrane protein targets
- Extensive experience in using of fully human transgenic mice
- Comprehensive immunization strategies including hock and repetitive immunization at multiple sites (RIMMS) are available
- Novel immune tolerance breaking technology for high homology and conservative antigens
- Extensive experience with genetic immunization with Gene Gun and in vivo electroporation
- Successful experience in antibody generation against multi-transmembrane domain proteins like GPCR/ion channel, high homology, and other challenging targets
- High throughput protein-based and cell-based screening platforms such as Mirrorball and Acumen are well established
- Well established binding, receptor-ligand blocking, and cell-based functional assays
| Target Class | Homology | Size of ECD | Immunization Strategy | # of Binders | # of Functional Clones |
| GPCR (C5aR) | >80% | 37+17+27+17 aa | DNA & Cell | 46 | 10 |
| GPCR Heterodimer | 98%/91% | 122 kDa + 90 kDa | Protein | 78 | 8 |
| GPCR | Undisclosed | Undisclosed | DNA + nanodisc/cell +nanodisc | 5 | 5 |
| GPCR | Undisclosed | 35+5+19+5 aa | Cell | 34 | 17 |
| GPCR (Apelin Receptor) | ~60% | 26+10+28+14 | Dendritic cell/cell | ~200 | ~15 |
| NKG2A | >90% homology | 17 kDa | Protein + Cell/DNA + Cell | 16 | 7 |
| TNFR Family | 96% | 50 aa | Protein/Cell | 29 | Only look for binders |
| Type I Transmembrane | 97% | ~70 kDa | DNA, Cell, Protein | 210 | 13 |
| RTK Family | 95% | ~66 kDa | Protein + Cell/DNA + Cell | 93 | 4 |
| Integrin Family | 93%/92% | 250 kDa | Protein, DNA | 37 | 17 |
| Ion Channel | 92%/60% | 13 + 34 aa | DNA + Protein/Cell + Protein/Protein | 88 | 14 |
| Multi-transmembrane | 87%/80% | 15 + 10 aa | Cell | 55 | Only look for binders |
| Multi-transmembrane | 100% ECL1 | 53 + 24 aa | DNA + Cell | 39 | 14 |
